四虎成年永久免费网站-四虎成人4hutv影院-四虎成人www国产精品-四虎成人国产精品视频-四虎成人精品-四虎成人精品国产一区a

您好,歡迎進(jìn)入上海力敏實(shí)業(yè)有限公司網(wǎng)站!
一鍵分享網(wǎng)站到:
您現(xiàn)在的位置:首頁 >> 產(chǎn)品中心 >> 生化試劑、抗體、血清 >> 常規(guī)生化試劑 >> 43815sigmaDL-二硫蘇糖醇DL-Dithiothreitol

sigmaDL-二硫蘇糖醇DL-Dithiothreitol

  • 更新時(shí)間:  2023-07-24
  • 產(chǎn)品型號(hào):  43815
  • 簡(jiǎn)單描述
  • sigmaDL-二硫蘇糖醇DL-Dithiothreitol
    for molecular biology
    product line
    BioUltra
    ≥99.5% (RT)
    total impurities
    DNases, none detected
    RNases, none detected
    insoluble matter, passes filter t
詳細(xì)介紹

sigmaDL-二硫蘇糖醇DL-Dithiothreitol

CAS number: 3483-12-3
Product Description:
Appearance: White Powder
Molecular formula: C4H10O2S2
Melting Point: 42-43°C
Molecular weight: 154.1 g/mol
Solubility: 0.1 M in H2O, 20°C, complete, colorless
pH: 4.0-6.5 (0.1 M in H2O, 25°C)
This product is designated as BioChemika Ultra for Molecular Biology grade and is suitable for applications in the molecular biology, specially if high purity is required. It has been analyzed for low contaminating impurity and the absence of nucleases.
DTT is synthetic. A method of preparation has been described in J. Chem. Soc. 253 (1949)
DTT is an agent for reducing -S-S- to -SH. Redox potential: -0.33 volts at pH 7. DTT acts as a protective agent for -SH groups of other species in solution. In this reaction the DTT is oxidized to the cyclic disulfide, and thereby ensures the reduction of other disulfides in solution. The disulfide reduction is complete in minutes at pH 8. A specific and sensitive assay for disulfide groups is based on determination of resulting monothiols with DTNB (Fluka 43760).2
Applications:
It is used in buffers for isolation of RNA5 or buffers used for RT-PCR starting with mRNA6,7.
Precautions and Disclaimer:
For Laboratory Use Only. Not for drug, household or other uses.
Preparation Instructions:
The solubility of DTT has been tested by Fluka at 50 mg/ml in water, resulting in a clear colorless solution. It has been described as freely soluble in water, ethanol, acetone, ethylate, chloroform and ether.
Solutions oxidize relatively slowly in air.3 It is recommended to make solutions fresh daily. Recorded half-life (hours) of DTT solutions at various pH and temperatures are as follows:4
Conditions (all in 0.1 M KPO4 buffer)
Half life (Hours)
pH 6.5 at 20°C
40
pH 7.5 at 20°C
10
pH 8.5 at 20°C
1.4
pH 8.5 at 0°C
11
pH 8.5 at 40°C
0.2
pH 8.5 at 20°C +0.1 mM Cu2+
0.6
pH 8.5 at 20°C +0.1 mM EDTA
4
Storage/Stability:
Storage Temperature: 4°C
When stored as indicated, this product is stable for years.

 


上海力敏實(shí)業(yè)有限公司代理sigma產(chǎn)品,咨詢sigmaDL-二硫蘇糖醇DL-Dithiothreitol 詳細(xì)產(chǎn)品信息


留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
主站蜘蛛池模板: av天天看| 国产秦先生大战白丝97在线 | 日日影院| 成人午夜短视频 | a级毛片在线免费观看 | 亚洲国产精品久久卡一 | 538国产在线 | 日韩a在线看免费观看视频 日韩v | 天天谢天天干 | 外国成人网在线观看免费视频 | 亚洲精品人成网在线播放影院 | 最新国产你懂的在线网址 | 91插视频| a视频免费观看 | 久久www免费人成看国产片 | 亚洲国产成人久久综合一 | 日韩欧美影视 | 18女人免费毛片a级 18女人水真多免费高清毛片 | avtt天堂网永久资源手机版 | 天天色天天插 | 亚洲成人中文 | 久久www免费人成_看 | 99视频在线精品免费观看18 | 久久精品视频99精品视频150 | 国产精品午夜久久 | 免费观看久久 | 天天做天天爱天天爽综合区 | 国产精品第一区在线观看 | 视频三区精品中文字幕 | 欧美中文在线 | 国产22页| 国产第一页福利 | 狠狠久久久久久亚洲综合网 | 国产亚洲视频网站 | 国产v精品成人免费视频400条 | 国产在线欧美精品 | 一本大道香蕉大69 | 久久久这里只有精品免费 | 久久激情网| 九九视频这里只有精品 | 女女女女女女bbbbbb级毛片 |