四虎成年永久免费网站-四虎成人4hutv影院-四虎成人www国产精品-四虎成人国产精品视频-四虎成人精品-四虎成人精品国产一区a

您好,歡迎進(jìn)入上海力敏實(shí)業(yè)有限公司網(wǎng)站!
一鍵分享網(wǎng)站到:
您現(xiàn)在的位置:首頁 >> 產(chǎn)品中心 >> 生化試劑、抗體、血清 >> 常規(guī)生化試劑 >> 43815sigmaDL-二硫蘇糖醇DL-Dithiothreitol

sigmaDL-二硫蘇糖醇DL-Dithiothreitol

  • 更新時(shí)間:  2023-07-24
  • 產(chǎn)品型號(hào):  43815
  • 簡(jiǎn)單描述
  • sigmaDL-二硫蘇糖醇DL-Dithiothreitol
    for molecular biology
    product line
    BioUltra
    ≥99.5% (RT)
    total impurities
    DNases, none detected
    RNases, none detected
    insoluble matter, passes filter t
詳細(xì)介紹

sigmaDL-二硫蘇糖醇DL-Dithiothreitol

CAS number: 3483-12-3
Product Description:
Appearance: White Powder
Molecular formula: C4H10O2S2
Melting Point: 42-43°C
Molecular weight: 154.1 g/mol
Solubility: 0.1 M in H2O, 20°C, complete, colorless
pH: 4.0-6.5 (0.1 M in H2O, 25°C)
This product is designated as BioChemika Ultra for Molecular Biology grade and is suitable for applications in the molecular biology, specially if high purity is required. It has been analyzed for low contaminating impurity and the absence of nucleases.
DTT is synthetic. A method of preparation has been described in J. Chem. Soc. 253 (1949)
DTT is an agent for reducing -S-S- to -SH. Redox potential: -0.33 volts at pH 7. DTT acts as a protective agent for -SH groups of other species in solution. In this reaction the DTT is oxidized to the cyclic disulfide, and thereby ensures the reduction of other disulfides in solution. The disulfide reduction is complete in minutes at pH 8. A specific and sensitive assay for disulfide groups is based on determination of resulting monothiols with DTNB (Fluka 43760).2
Applications:
It is used in buffers for isolation of RNA5 or buffers used for RT-PCR starting with mRNA6,7.
Precautions and Disclaimer:
For Laboratory Use Only. Not for drug, household or other uses.
Preparation Instructions:
The solubility of DTT has been tested by Fluka at 50 mg/ml in water, resulting in a clear colorless solution. It has been described as freely soluble in water, ethanol, acetone, ethylate, chloroform and ether.
Solutions oxidize relatively slowly in air.3 It is recommended to make solutions fresh daily. Recorded half-life (hours) of DTT solutions at various pH and temperatures are as follows:4
Conditions (all in 0.1 M KPO4 buffer)
Half life (Hours)
pH 6.5 at 20°C
40
pH 7.5 at 20°C
10
pH 8.5 at 20°C
1.4
pH 8.5 at 0°C
11
pH 8.5 at 40°C
0.2
pH 8.5 at 20°C +0.1 mM Cu2+
0.6
pH 8.5 at 20°C +0.1 mM EDTA
4
Storage/Stability:
Storage Temperature: 4°C
When stored as indicated, this product is stable for years.

 


上海力敏實(shí)業(yè)有限公司代理sigma產(chǎn)品,咨詢sigmaDL-二硫蘇糖醇DL-Dithiothreitol 詳細(xì)產(chǎn)品信息


留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
主站蜘蛛池模板: 多人伦交性欧美精品欧 | 免费人成视网站在线观看不卡 | 久久草在线精品 | 亚洲日本欧美产综合在线 | 天天影视欲香欲色成人网 | 欧美日韩一日韩一线不卡 | 欧美一级免费观看 | 国产操老逼 | 爱搞逼综合 | 亚洲综合日韩在线亚洲欧美专区 | 亚洲欧美日韩在线线精品 | 四虎黄色网址 | 伊人蕉久中文字幕无码专区 | 亚洲精品久久77777 | 日本xxxx色视频在线观看 | 在线观看国产一区二区三区 | 国产欧美亚洲精品一区 | a级毛片在线| 国产精品日产三级在线观看 | 久精品在线 | 日本三级黄色片网站 | 四虎国产精品永久地址48 | 欧美视频一区二区专区 | 在线观看日韩www视频免费 | 国产精品成人观看视频免费 | 伊人精品视频在线观看 | 国产精品夜色7777青苹果 | 亚洲视频中文字幕在线观看 | 草碧网 | 精品伊人久久香线蕉 | 国产极品嫩模大尺度福利视频 | 日本中文字幕视频 | 欧美国产日韩在线观看 | 美国一级大黄香蕉片 | 国产夜夜春 | 久草免费在线 | 国产黄a三级三级三级 | 亚洲欧洲日产国码二区首页 | 欧美日本高清一本二本三本 | 中文字幕在线最新在线不卡 | 日日艹 |